The quality of drinking water resources is critical for public health, particularly due to the potential entry of pollutants and nutrients that can lead to bioaccumulation and biodiversity risks. This study focuses on the Yedgaon Dam, an earthfill and gravity dam on the Kukadi River near Junnar, Pune district, Maharashtra, India, primarily serving irrigation purposes. The water from Yedgaon Dam is vital for agricultural activities in Yedgaon village. A comprehensive physico-chemical and microbiological analysis was conducted to assess the suitability of this water for irrigation. Parameters such as color, pH, temperature, nitrite, nitrate, total dissolved solids (TDS), total suspended solids (TSS), dissolved oxygen (DO), biological oxygen demand (BOD), chemical oxygen demand (COD), total hardness, phosphate, alkalinity, salinity, chloride, sulphite, sulphate, calcium, magnesium, phosphorus, and microbiological indicators were measured. The findings indicate that all parameters fall within the standard ranges specified by the World Health Organization (WHO), affirming the water’s suitability for irrigation purposes.