Professional stress refers to the harmful physical and emotional effects on the individual when job requirements do not match workers’ resources or needs. Work stress can lead to poor mental and physical health. Therefore, the aim of this study was to assess the Professional stress of Dietician / nutritional professionals. In this study, the survey method was adopted using a stress scale (SS) developed by the investigator (2022). The population was fifty Dietician in Southern regions of Tamilnadu. Percentage analysis and ‘t’ test were used for data analysis. The findings of the study revealed that the Dietician have moderate level of Professional stress. Unmarried Dietician have more stress when compared to married health care workers. There is significant difference among professional stress of Dietician of different age, educational qualification and work experience. Therefore, needs to set intervention programs focusing on prevention and control of stress is mandatory.