The plant kingdom for its therapeutic use has been inadequately explored and there is a tremendous scope for the drug discovery for the human health problems. India has rich in plant wealth and also having excellent usage of medicinal plants with Ayurvedic science. The objective of this study was to carry compound class detection present in extract of Alysicarpus rugosus (Wild.) using chromatographic and by Phytochemical screening methods. Preliminary phytochemical screening was done, physical constants were evaluated and HPTLC studies were carried out. Preliminary phytochemical screening and HPTLC analysis of the extract showed the presence of Tannins, Phenolic compounds and flavonoids. It can be concluded that HPTLC analysis of extract can be used as an authentic diagnostic tool for the correct identification of secondary metabolites and it is useful as a phytochemical marker and also a good estimator of genetic variability in plant populations.