Objective: The effects cold stress or diabetes caused pathophysiology are well known. This study aims not only to access the effects of cold stress but also its interaction with diabetes. The adverse effects of diabetes and cold stress on reproductive impairments is considered as a co-morbid factor for aggravating diabetic complications, since their co-exposure results in synergistic effects. The aim of this study was to address the extent of reproductive impairments occurred in co-exposed rats and the amelioration brought by ethanol extract of Tribulus terrestris fruit (TTF). Methods: Female Wistar rats of induced diabetes (STZ 45mg/kgbw) were exposed to cold stress (4±2°C, 3hr/day for 7 days) and were supplemented with TTF extract (200 mg/kgbw/day for 21 days) to investigate the impaired reproductive performance. Results: A significant change was evident in reproductive cyclicity among the positive control groups studied. Following hyperglycemic state, the estrous pattern and levels of reproductive hormones changed significantly (P<0.05) in both diabetic as well as in co-exposed groups. Further, supplementation of TTF extract was found beneficial in ameliorating the alterations induced upon co-exposure. Conclusion: Synergistic effects of co-exposure witnessed in the functional tissues of female reproductive tract which found to disrupt the reproductive cyclicity by inhibiting estrogen and progesterone secretion. The polyphenol rich, TTF extract has the ability to quench free radical generated oxidative stress by enhancing the status of antioxidant enzymes thereby enhance endocrine functioning, to normalize reproductive ability in co-exposed rats.