Molecular Characterization and Genetic Diversity Analysis of Commercial Soybean (Glycine Max L. Merr.) Genotypes with SSR Marker

Abstract

Soybean [Glycine max (L.) Merrill] is the world’s most economically important legume crop it provides high-quality nutrients with the richest amount of protein. The current study is based on molecular markers, Simple Sequence Repeats (SSRs) to determine the genetic diversity of genotypes of soybeans. Two varieties viz., AMS 155 and CSB 09-03 exhibited a score of nine (9) in both years thus providing the highly susceptible varieties. Among the polymorphic SSR markers, the highest genetic diversity was observed with Satt256 (0.6948) while the lowest genetic diversity was observed with Satt299 (0.4179). Similarly, polymorphism information content (PIC) was highest (0.8274) in Satt114 and lowest (0.3729) in Satt070 among all polymorphic markers used for the identification of genetic diversity. These results advise that the uses of SSR markers are very efficient for measuring genetic diversity and relatedness as well as identifying soybean varieties. Genetic materials (DNA & RNA) may be used for the improvement of soybean genotypes genetically to increase productivity in the country.