Formulation, Optimization And Characterization Of Folate-Conjugated Polymeric Nanoparticles Of Paclitaxel

Abstract

The rationale of present study is to formulate and characterize folate-conjugated poly(lactide-co-glycolide)-block-poly(ethyleneglycol)(PLGA-PEG-FOL) nanoparticles loaded with Paclitaxel . Folate conjugated nanoparticles can be used as vehicles to assist active targeting of various anti- cancer drugs to target cancer cells. PLGA–PEG–FOL was synthesized as follows: activation of PLGA, conjugation of activated PLGA with PEG-bis-amine, and conjugation of FA to PLGA–PEG. Characterization of the synthesized conjugate was done by using FTIR and 1HNMR. Drug loaded nanoparticles was prepared by the Double emulsion solvent evaporation(DESE). After the formulation of polymeric nanoparticles, various parameters like particle size, polydispersity index and zeta potential were measured. Morphology was observed using scanning electron microscopy. Flow Cytometry was used to evaluate the uptake of optimized Folate conjugated NPs by the breast cancer cells (MCF7). The results showed that FITC labelled PPF-NPs were internalized into the MCF7 cells effectively.