A Study on Isolation and Characterization of Dye Degrading Microorganism

Abstract

Over the past decades, biological decolorization has been investigated as a method to transform, degrade or mineralize azo dyes. Moreover, such decolorization and degradation is an environmentally friendly and cost competitive alternative to chemical decomposition possess. Therefore, present study was aimed for isolation and identification of dye degrading microorganisms. The effluent from textile industry was screened for dye decolorization potential. Further, morphological and biochemical characterization was carried out. Results revealed that three isolates viz. IS 1, IS 2, and IS 3 shown maximum decolorization percentage at 150 mg/mL concentration. However, among the three isolates (IS 1, IS 2, and IS 3), IS 2 shown maximum decolorization potential of selected dyes at the concentration of 150 mg/mL, and hence IS 2 was selected for further morphological and biochemical characterization studies. IS 2 was positive for catalase, oxidase, methyl red, Voges Proskauer, nitrate utilization, urease activity, casein hydrolysis, and starch hydrolysis tests. Whereas, the isolated strain (IS 2) was negative for indole, citrate utilization, and CAMP tests. The isolated bacterial strain (IS 2) was grown on Bacillus cereus agar plan, and hence IS 2 strain was identified as Bacillus sp. In conclusion, newly isolated Bacillus sp. could be used as a good tool for bioremediation of various textile effluent treatment by converting toxic dye into colorless, harmless product., the treated effluents can be reused for textile industry.