In Vitro Cytotoxicity Assessment of HepG2 Cells of Pulicaria jaubertii Potential via MTT Assay

Abstract

The search for novel anticancer agents from natural sources continues to be a promising avenue in cancer research. Pulicaria jaubertii, a medicinal plant with traditional therapeutic applications, has shown potential bioactive properties warranting investigation for anticancer activity. This study aimed to evaluate the in vitro cytotoxicity of Pulicaria jaubertii extracts against HepG2 hepatocellular carcinoma cells using the MTT assay to assess its anticancer potential. The plant material was extracted using methanol, petroleum ether, and aqueous solvents. Cytotoxicity was assessed using the MTT assay. The results demonstrated that the methanol extract exhibited the most potent cytotoxic activity with an IC₅₀ value of 87.48 µg/mL. In contrast, petroleum ether and aqueous extracts showed significantly lower activity, with IC₅₀ values of 283.3 µg/mL and 287.5 µg/mL, respectively. These findings suggest that P. jaubertii contains bioactive compounds, particularly in the methanolic fraction, warranting further investigation for anticancer drug development.