Evidence for the creation of the M412 intermediate by Raman Spectroscopy

Abstract

To "trap" the M intermediate, bcbR crystals were subjected to both photo-excitation and dehydration. Resonance In this chapter, the presence of the M intermediate is exploited as a cue to search for traces of a photocycle using Raman spectroscopy of single hydratedbcbR crystals. Resonance Before using bacteriorhodopsin, Raman spectroscopy is first explained.