Preliminary Phytochemical & Anti-diabetic Activity of Leaves and Flowers of Rungia repens Nees

Abstract

The aim of the present investigation is to study the Preliminary Phytochemical & Anti-diabetic Activity of Leaves and Flowers of Rungia repens Nees. MATERIAL & METHODS- The leaves and flowers of Rungia repens Nees were collected from outfield and also purchased from local markets during the month of July that shows the green color with rough surface. The correctly identified plant leaves and flowers were dried in shade at room temperature & after 4-5 days, it is formed in powder by mixer grinder. The mare was dried in air to remove traces of petroleum ether. Defatted drug was subjected to extraction with chloroform, ethyl acetate, ethanol, methanol and aqueous in soxhlet apparatus, the extraction was completed in 17-18 hrs. Preliminary phytochemical screening was performed for the presence of various active phytochemicals. Acute oral toxicity test was carried out according to the OECD guideline No. 423. After fasting 18 hours, the rats were injected intraperitoneal injection through tail vein with a single dose of 40 mg/kg Streptozocin (Sigma, St. Louis, Mo, USA), freshly dissolved in citrate buffer (pH 4.5). The test drug and reference drug was administered orally at two dose level for a period of 21 days from starting day of diabetes. RESULTS- The study on selected plant material, shows, that the difference of two consecutive weighing after drying for 30min. and cooling for 30 min. in a desiccators- 0.09 & 0.23 gm. The Experimental results on selected plant material shows – volume occupied by 1 gm of plant material= 1.62, 1.45 ml. Petroleum ether, chloroform, ethyl acetate, ethanolic extracts had moderately significant effects (p