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RESEARCH PAPER
Year : 2019  |  Volume : 8  |  Issue : 3  |  Page : 29-38

Bioconversion of phenolic acids by probiotic lactic acid bacteria


1 Department of Five Years Integrated Course in Bioanalytical Sciences, GNIRD, Guru Nanak Khalsa College, Matunga, Mumbai 19, India
2 National Facility for Biopharmaceuticals, Guru Nanak Khalsa College, Matunga, Mumbai 19, India

Correspondence Address:
Kavita Pandey
Department of Five Years Integrated Course in Bioanalytical Sciences, GNIRD, Guru Nanak Khalsa College, Matunga, Mumbai 19
India
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Source of Support: None, Conflict of Interest: None


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Increased levels of accumulation of toxic waste products in environment, demands for bioconversion. The advantages of bioconversion process have been well established over chemical technologies, moreover, no hazardous by-products are generated. Literature advocates tremendous scope of bioconversion in pharmaceutical industries. Phenolic compounds are important substances with widespread occurrence in plants and therefore in the human diet, known for several their beneficial influence on human metabolism. However, bioconversion of these compounds results in the formation of high value-added and costly industrial metabolites with enhanced bioactivity. Many benign microbes that are capable of degrading phenolic acids have been isolated in the last few decades, with the objective of screening an established probiotic (preferably Lactobacillus strain) which is used in the food industry. Bioconversion of phenolic acids gives valuable products like phenolic glycosides. This work is an attempt to screen and select LAB for their phenolic acid degrading capabilities and carryout bioconversion studies with the the most effective strain(s) followed by characterization. The idea was to primarily determine the bioconversion efficiency of isolated bacterial probiotic species, followed by identification of products formed during bioconversion using analytical tools TLC and HPLC. Finally, shake flask optimization was carried out for maximizing bioconvertion of phenolic acids.


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