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Year : 2015  |  Volume : 4  |  Issue : 1  |  Page : 120-125

Supercritical carbon dioxide (SC-CO2) extraction of bioactive compounds from mulberry (Morus Alba L) leaves: Process optimization

1 Department of Processing and Food Engineering, College of Technology and Engineering, M.P.U.A.T., Udaipur, Rajasthan, India
2 Department of Processing and Food Engineering, College of Agricultural Engineering, University of Agricultural Sciences, Raichur, Karnataka, India

Correspondence Address:
V Ramya
Department of Processing and Food Engineering, College of Technology and Engineering, M.P.U.A.T., Udaipur, Rajasthan
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Source of Support: None, Conflict of Interest: None

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ABSTRACT A simple, more efficient, less time consuming extraction technique has been developed for extraction of rutin, a flavonoid and total phenolics from dry leaf powder of M. alba using supercritical carbon dioxide (SC-CO2) equipment. Extraction parameters such as temperature (40, 50 and 60°C), pressure (100, 150 and 200 bar) and dynamic extraction time (40, 60 and 80 min) were optimized using a factorial design (33). The best conditions obtained for SC-CO2 extraction of flavonoids and phenolics were found within the experimental range of variables at a temperature of 50°C, pressure of 200 bar and dynamic extraction time of 80 min. High performance liquid chromatography (HPLC) was used to determine the activity of the rutin from mulberry leaf powder extract. Apart from the optimum SFE condition, the other extraction condition at minimum (100 bar, 40°C and 40 min) and maximum (200 bar, 60°C and 80 min) level of each studied parameters were selected and analyzed by spectrophotometer.

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