A common annual weed that can be transmitted by people, animals, or water is Xanthium strumarium. Although its exact origin is still unknown, cocklebur may be a native species of California. Although it can be found in a range of waste locations, it is most prevalent in wet open sites. Periodic surveys of different forest locations were carried out in Raipur. Any leaves exhibiting disease symptoms on X. strumarium plants were gathered, and all emerging fungus were isolated. Twelve distinct fungal infections were isolated and identified from a variety of X. strumarium illness samples. From this weed, around 12 fungal isolates were found. Among these, Alternaria alternate did the most harm to the test plants. Since Alternaria alternate was shown to be the most virulent strain due to its phytotoxicity, so, it was selected as a biological agent for the management of the noxious weed X. strumarium.